The Growth of Isolated Cotyledons of Cucurbita Pepo
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چکیده
منابع مشابه
Isolation and Purification of Nuclear Dna from Excised Cotyledons of Cucurbita Pepo
High molecular weight genomic DNA was isolated from excised marrow cotyledons (Cucurbita pepo L., zucchini) using high concentration of ethidium bromide (400 μg.ml–1) in the grinding buffer and phenol deproteinization of the crude nuclear pellet. This preparation of DNA was free of proteins but contained polysaccharide contaminations which did not allow complete digestion with a number of restr...
متن کاملComplex spatial responses to cucumber mosaic virus infection in susceptible Cucurbita pepo cotyledons.
Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the h...
متن کاملMetabolic Requirement of Cucurbita pepo for Boron.
Lateral roots of intact summer squash seedlings (Cucurbita pepo L.) were used to quantify the effects of boron deficiency on DNA synthesis, protein synthesis, and respiration. The temporal relationship between changes in these metabolic activities and the cessation of root elongation caused by boron deprivation was determined. Transferring 5-day-old squash seedlings to a hydroponic culture medi...
متن کاملl-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.
ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are 4.7 mm and 6.3 mm, respectively. The enzyme has a molecular weight of 48,000 ...
متن کاملDistribution of a - Galactosidase in Cucurbita pepo
The distribution of a-galactosidase (a-D-galactoside galactohydrolase IEC 3.2.1.221) in Cucurbita pepo has been determined in an attempt to assess its involvement in hydrolysis of transport sugars of the raffinose oligosaccharide series (la-1-6-galactopyranosyllj sucrose). Extracts prepared from leaves and petioles at different stages of development, roots, flowers, dry and germinating seeds, a...
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ژورنال
عنوان ژورنال: Annals of Botany
سال: 1941
ISSN: 1095-8290,0305-7364
DOI: 10.1093/oxfordjournals.aob.a087387